Key Technical Points of Shaking Culture in Edible Fungi Optimization of flask liquid fermentation medium for xylanase producing strain Pichia pastoris 食用菌液体菌种摇瓶培养操作技术要点毕赤酵母摇瓶产木聚糖酶发酵条件的优化
The fermentation culture medium and shaking flask fermentation conditions of glutathione have been researched by using orthogonal experiments with Saccharomyces cerevisiae. 通过正交试验,研究了啤酒酵母摇瓶发酵生产谷胱甘肽的培养基的组成以及发酵培养条件。
The simplest suspension culture system is a conical flask gently shaken on the platform of an orbital incubator. 最简单的悬浮培养系统是一个在摇床平板上轻微摇晃的锥形烧瓶。
Methods: Isolate and purified the MSCs from human fetal bone marrow based on the different growth characteristic of attaching to the wall of cell culture flask. 方法:利用细胞差速贴壁生长特性分离纯化人胎骨髓间充质样干细胞;利用流式细胞仪检测其细胞周期和表面标志;
Optium fermentation medium and culture conditions were determined through Erlenmeyer flask liquid fermentation, ampliate liquid fermentation technique was also determined later. 通过茯苓的摇瓶液体发酵培养,确定了茯苓液体发酵的最适发酵培养基和最适培养条件,并进一步确定了最佳的放大培养工艺。
Determination of Bacterial Cell Population for Hydrocarbon-Degrading Bacteria by Using Culture Flask Test 细菌瓶法用于石油烃降解菌菌数测定
The culture flask were inflated with pure nitrogen gas to cause the cells hypoxia, to observe the induced effects of hypoxia on NOS; 用充纯氮气的方法造成细胞缺氧,观察缺氧对NOS的诱导作用;
Methods MSCs were obtained from the tibia and femur bone marrow of SD rats and cultured by their adherence characteristics to culture flask. 方法利用SD大鼠间充质干细胞(取自股骨和胫骨)贴壁生长的特性,培养纯化,传代扩增。
A culture flask for determining bacterial cell population of surfactant producing bacteria ( SPB) is prepared. 研制了测量产表面活性剂菌(SPB)菌数用的测试瓶。
Morphology observation of the cell: at the state of culturing, observe the morphology of cell of experiment groups and control in culture flask by invert microscope. 细胞形态学观察:在培养状态下,用倒置显微镜观察培养瓶内实验组及对照组细胞形态变化情况。
The culture conditions were established in shake flask with the index of dry mycelium. 以干菌丝体得率为指标,对影响发酵产量的重要因子设计正交试验,得出最佳培养条件。
2D dynamic culture group: The cells were inoculated directly in a culture flask in RCCS for a dynamic culture under microgravity. 二维动态培养组:细胞直接接种于平面培养瓶,置于旋转式细胞培养系统,微重力条件下动态诱导。
The CD41~+ cell content decreased at the late stage of culture in spinner flask but increased all the time in T-flask. 转瓶培养中CD41+细胞含量到后期出现下降,而方瓶中则一直增加。
CFU-Mk cell content reached a peak value at the 7~ ( th) day after culture in spinner flask and at the 10~ ( th) day in T-flask, and its content in spinner flask was significantly lower than that in T-flask all the time. CFUMK则不同,其含量在转瓶培养第7天时达到最高,而在方瓶第10天达到最高,在转瓶中CFUMK的含量一直低于方瓶培养;
Results: BM-MSCs grew into monolayer cells attached on the bottom of culture flask. 结果:BM-MSC为单层贴壁生长,呈现规则的集束辐射状排列。
2D static culture group: The cells were inoculated directly in a plane culture flask for static culture. 二维静态培养组:细胞直接接种于平面培养瓶静态培养。
Esterifying enzyme activity of Rhizopus sp. was affected by the inoculating volume, culture density, flask of fermentation volume, initial pH value, temperature and agitation frequency. 在液态培养条件下,接种量、装液量、初始pH值、温度、摇床转速等因素影响根霉酯化酶的酶活。影响产酶的主要因素是温度、转速。
A simple and convenient culture flask test is recommended instead. 本文推荐使用简单易行的细菌瓶法。
About 2 weeks later, a unilaminar cells formed at the bottom of culture flask, presenting cluster-shape. 约2周后,细胞培养瓶底可形成一单层细胞,呈集簇状生长。
Methods Bone marrow was collected from femur and tibia of 2 month old male SD rats and seeded in cell culture flask. 方法取2月龄SD大鼠胫骨和股骨骨髓进行体外培养。
This strain was selected for further optimization of the culture and induction conditions in shake flask and fermentor. 分别在摇瓶和发酵罐中进行了培养条件和诱导条件的优化。
Morphology of tumor cells in tissue culture flask in MEL cells than change. 组织中肿瘤细胞形态较培养瓶中的MEL细胞有所改变。